[1]任 崴,黄廷林.西北某市地下水源真菌种群及多样性特征分析[J].西安建筑科技大学学报(自然科学版),2021,(02):295-301.[doi:10.15986/j.1006-7930.2021.02.020]
 REN Wei,HUANG Tinglin.Analysis of fungi population and diversity characteristics of groundwater source in a city of northwest China[J].J. Xi’an Univ. of Arch. & Tech.(Natural Science Edition),2021,(02):295-301.[doi:10.15986/j.1006-7930.2021.02.020]





Analysis of fungi population and diversity characteristics of groundwater source in a city of northwest China
任 崴12黄廷林1
(1.西安建筑科技大学 环境与市政工程学院 陕西省环境工程重点实验室,陕西 西安 710055; 2.河南科技大学 建筑学院,河南 洛阳 471023)
REN Wei12 HUANG Tinglin1
(1.Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering,Xi’an Univ. of Arch.& Tech., Xi’an 710055, China; 2.School of Architecture, Henan University of Science and Technology, Luoyang 471023, China)
地下水 真菌多样性 DGGE
ground water fungal diversity DGGE
饮用水中的真菌污染会引起水体产生嗅味,其潜在致病性容易导致流行性疾病的发生.以西北某市水源井水为研究对象,利用PCR-DGGE分子指纹图谱技术分析水体中的真菌多样性.研究得出,该地区地下水中真菌种类较为丰富,真菌多样性指数、丰富度指数和均匀度指数分别为0.89~1.33、9~25 和0.405~0.460. 不同样点真菌群落相似性存在一定关系,相邻样点间相似性较高.对DGGE条带进行回收、克隆、测序,显示隐球菌属(Cryptococcus.sp)、暗球腔菌属(Pluteus.sp)、镰刀菌属(Fusarium.sp)、青霉属(Penicillium.sp)四种类型为优势种属.相关性分析表明不同种属的真菌与环境变量的相关性并不相同,pH值和温度和对镰刀菌属(Fusarium.sp)、青霉属(Penicillium.sp)的生长相关性较大. 研究为地下水真菌污染控制提供了一定的理论依据.
The occurrence of fungi in drinking-water may be linked to taste, odour and some health problems, and its potential pathogenicity can easily lead to the occurrence of epidemic diseases. Water samples were taken from twelve ground water source wells, then PCR-DGGE molecular fingerprinting technology was used to analyze the fungal diversity. The results showed that there were abundant fungal species in groundwater in this area, with fungal diversity index, richness index and evenness index ranged from 0.89~1.33, 9~25 and 0.405~0.460, respectively. There was a certain relationship between the similarity of fungal communities in different sites, and the similarity between adjacent spots was higher. The DGGE band was recovered, cloned, and sequenced, and the results showed that Cryptococcus sp., Pluteus sp., Fusarium sp., Penicillium sp. Species types were dominant species. Correlation analysis showed that the correlation between different species of fungi and environmental variables was not the same, pH and temperature are more relevant to the growth of Fusarium sp. and Penicillium.sp. The research provides a certain theoretical basis for the control of groundwater fungal pollution.


[1]HAGESKAL G, LIMA N, SKAAR I. The study of fungi in drinking water[J].Mycological Research, 2009, 113(2): 165-172.
[2]GARRETT L. World health statistics 2008[M].Geneva:World Health Organization, 2009.
[3]KANZLER D, BUZINA W, PAULITSCH A, et al. Occurrence and hygienic relevance of fungi in drinking water[J].Mycoses, 2008, 51(2): 165-9.
[4]PEREIRA V, FERNANDES D, CARVALHO G, et al. Assessment of the presence and dynamics of fungi in drinking water sources using cultural and molecular methods[J].Water Research, 2010, 44(17): 4850-4859.
[5]DOGGETT M S. Characterization of fungal biofilms within a municipal water distribution system[J].Applied and Environmental Microbiology, 2000, 66(3): 1249-1251.
[6]BUCHELI T D, WETTSTEIN F E, HARTMANN N, et al. Fusarium mycotoxins: overlooked aquatic micropollutants?[J].Journal of Agricultural and Food Chemistry, 2008, 56(3): 1029-1034.
[7]黄廷林, 赵建超, 文刚, 等. 臭氧-氯顺序灭活地下水源水中真菌的效能[J].环境工程学报, 2016(10): 4691-4697.
HUANG Tinglin, ZHAO Jianchao, WEN Gang, et al. Sequential inactivation of fungi in drinking groundwater by ozone and chlorine[J].Chinese Journal of Environmental Engineering, 2016(10): 4691-4697.
[8]韩世忠, 高人, 马红亮, 等. 建瓯万木林自然保护区两种森林类型土壤真菌多样性[J].生态学杂志, 2015, 34(9): 2613-2620.
HAN Shizhong, GAO Ren, MA Hongliang, et al. Soil fungal diversities of two types of forests in Jian’ou Wanmulin Nature Reserve[J].Chinese Journal of Ecology, 2015, 34(9): 2613-2620.
[9]黄廷林, 邸诗雨, 张海涵,等. 西安市典型景观水体水质与微生物种群结构多样性[J].应用与环境生物学报, 2014, 20(4): 697-703.
HUANG Tinglin, DI Shiyu, ZHANG Haihan, et al. Water quality and diversity of microbial community structure of Xi’an typical scenic water bodies[J].Chinese Journal of Applied & Environmental Biology, 2014, 20(4): 697-703.
[10]AL-GABR H M, ZHENG T, YU X. Occurence and quantification of fungi and detection of mycotoxigenic fungi in drinking water in Xiamen City, China[J].Science of The Total Environment, 2013, 466(467):1103-1111.
[11]ANAISSIE E J.Pathogenic molds(including Aspergillus species)in hospital water distribution systems: a 3-year prospective study and clinical implications for patients with hematologic malignancies[J].Blood, 2003,101(7):2542-2546.
[12]G?TTLICH E, VAN DER LUBBE W, LANGE B, et al. Fungal flora in groundwater-derived public drinking water[J].International Journal of Hygiene and Environmental Health, 2002, 205(4): 269-729.
[13]LUO H F, QI H Y, XUE K, et al. A preliminary application of PCR-DGGE to study microbial diversity in soil[J].Acta Ecologica Sinica, 2003,23(8):1570-1575.
[14]AMANN, RUDOLF, I., et al. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J].Microbiological Reviews, 1995,
[15]孙宝盛, 张斌, 吴卿, 等. 应用PCR-DGGE技术解析MBR中微生物群落多样性[J].天津大学学报:自然科学与工程技术版, 2008, 41(3): 356-361.
SUN Baosheng, ZHANG Bin, WU Qing, et al. Application of PCR-DGGE to analysis of microbial community diversity in MBRs[J].Journal of Tianjin University, 2008, 41(3): 356-361.
[16]王风芹, 侯淑芬, 谢慧,等. 贾鲁河水体微生物菌群结构季节动态变迁研究[J].河南农业大学学报, 2012, 46(4): 438-441.
WANG Fengqin, HOU Shufen, XIE Hui, et al. Research on seasonal dynamic change of microbial population structure in Jialu River[J].Journal of Henan Agricultural University, 2012, 46(4): 438-441.
[17]宣淮翔, 安树青, 孙庆业,等. 太湖不同湖区水生真菌多样性[J].湖泊科学, 2011, 23(3): 469-478.
XUAN Huaixian, AN Shuqin, SUN Qingye, et al. Diversity of aquatic fungi in different areas of Lake Taihu[J].Journal of Lake Sciences, 2011, 23(3): 469-478.
[18]张利兰. 自然湿地水体中微生物多样性的研究[D].新乡:河南师范大学, 2011.
ZHANG Lilan. Study on the mirobial diversity in the water envirenments of natural wetland.[D].Xinxiang:Henan Normal University, 2011.
[19]赵建超. 复合污染地下水供水系统中丝状真菌控制研究[D].西安:西安建筑科技大学, 2016.
ZHAO Jianchao. Study on the control of filamentous fungi in fomposite polluted groundwater supply system[D].Xi’an:Xi’an Univ. of Arch. & Tech., 2016.
[20]王帅. 涠洲终端采油废水活性污泥中微生物群落结构分析[D].桂林:桂林理工大学, 2014.
WANG Shuai. The analysis of microbial communities among activated sludge from terminal petroleum produced wastewater in Weizhou[D].Guilin:Guilin University of Technology, 2014.
[21]邸诗雨. 典型微污染水体微生物种群结构及贫营养好氧反硝化细菌脱氮性能研究[D].西安:西安建筑科技大学, 2014.
DI Shiyu. Typical micro-polluted water and poor nutrition microbial community structure of aerobic denitrifying Bacteria denitrification performance[D].Xi’an:Xi’an Univ. of Arch. & Tech., 2014.
[22]WU Z L, GUO W Y, QIU J Z, et al. Cloning and localization of vip3A gene of Bacillus thuringiensis[J].Biotechnology Letters, 2004, 26(18): 1425-1428.
[23]吴雪梅. 乌伊岭湿地水生真菌多样性的研究[D].哈尔滨:东北林业大学, 2010.
WU Xuemei. Preliminary studies on diversity of freshwater fungi in wuyiling wetland[D].Harbin:Notheast Forestry University, 2010.
[24]GTTLICH E, LUBBE W V D, LANGE B, et al. Fungal flora in groundwater-derived public drinking water[J].International Journal of Hygiene & Environmental Health, 2002, 205(4): 269-279.
[25]MOH.AL-GABR H, YE C, ZHANG Y, et al. Effects of carbon, nitrogen and pH on the growth of Aspergillus parasiticus and aflatoxins production in water[J].Journal of Environmental Biology, 2013, 34(2spec.): 353-8.
[26]FRIEDRICH V W, G?BEL U B, ERKO S. Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis[J].Fems Microbiology Reviews, 1997,(3): 213-229.
[27]接伟光, 蔡柏岩, 葛菁萍, 等. 黄檗丛枝菌根(AM)真菌PCR-DGGE条件研究[J].黑龙江大学(自然科学学报), 2008, 25(4): 534-538.
JIE Weiguang, CAI Baiyan, GE Jingping, et al. Studies on PCR-denaturing gradient gel electrophoresis conditions of arbuscular mycorrhizal fungi of Phellodendren amurense Rupr.[J].Journal of Natural Science of Heilongjiang University, 2008, 25(4): 534-538.
(编辑 桂智刚)


收稿日期:2020-10-18 修改稿日期:2021-03-15
基金项目:国家自然科学基金资助项目(51979217,51978557); 陕西省重点研发计划项目(2018ZDXM-SF-029)
第一作者:任 崴(1979-),男,博士生,主要研究饮用水处理技术. E-mail:renwei_lz@163.com
通信作者:黄廷林(1962-),男,教授,博士生导师,主要研究方向为水源水质污染与富营养化控制. E-mail:huangtinglin@xauat.edu.cn
更新日期/Last Update: 2021-04-28