[1]王 琰,聂麦茜,王 菲,等.施氏假单胞菌N2降解1-羟基-2萘甲酸的特性及机理研究[J].西安建筑科技大学学报(自然科学版),2017,49(01):135-140.[doi:10.15986/j.1006-7930.2017.01.022]
　WANG Yan,NIE Maiqian,WANG Fei,et al.Study on the Characteristics of 1-hydroxy-2-naphthoic acid degradation by P. stutzeri N2[J].J. Xi’an Univ. of Arch. & Tech.(Natural Science Edition),2017,49(01):135-140.[doi:10.15986/j.1006-7930.2017.01.022]
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施氏假单胞菌N2降解1-羟基-2萘甲酸的特性及机理研究()
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西安建筑科技大学学报（自然科学版）[ISSN:1006-7930/CN:61-1295/TU]

卷:
49
期数:
2017年01期
页码:
135-140
栏目:
出版日期:
2017-03-03

文章信息/Info

Title:
Study on the Characteristics of 1-hydroxy-2-naphthoic acid degradation by P. stutzeri N2
文章编号:
1006-7930(2017)01-0135-06
作者:
王 琰1; 2; 聂麦茜1; 王 菲1; 聂红云1; 赵碧洁1
(1. 西安建筑科技大学环境与市政工程学院,陕西 西安 710055;2. 陕西省微生物研究所,陕西 西安 710043)
Author(s):
WANG Yan1; 2;  NIE Maiqian1;  WANG Fei1;  NIE Hongyun1;  ZHAO Bijie1
(1. School of Environmental and Municipal Engineering, Xian Univ. of Arch. & Tech. , Xian 710055,China; 2. Institute of Microbiology, Shaanxi Province Academy of Sciences, Xian 710043,China)
关键词:
含氧多环芳烃; 施氏假单胞菌; 1-羟基-2萘甲酸; 转化产物; 降解途径
Keywords:
Oxygen-containing Polycyclic Aromatic Hydrocarbons;  Pseudomonas stutzeri;  1-hydroxy-2-naphthoic acid;  transformation products;  metabolic pathways
分类号:
X132
DOI:
10.15986/j.1006-7930.2017.01.022
文献标志码:
A
摘要:
1-羟基-2-萘甲酸被认定为微生物修复多环芳烃（PAHs）污染过程中最易积累的一种含氧多环芳烃（OPAHs）中间产物，其对大多微生物有较强毒性，并且该物质的积累会抑制微生物对PAHs污染环境的成功修复．本文以施氏假单胞菌N2为对象，研究该菌株对1-羟基-2-萘甲酸的降解代谢能力和特性，并利用GC-MS对降解过程中积累的OPAHs进行鉴定和分析．研究结果表明，N2菌能够以50 mg/mL1-羟基-2-萘甲酸为唯一碳源和能源生长，并在以辛烷为共代谢碳源时其生长量和1-羟基-2-萘甲酸的降解率均可提高20 %．通过对9种主要中间代谢产物的GC-MS分析，推测脱羧是N2菌降解1-羟基-2-萘甲酸的主要途径．该研究结果将为解决PAHs污染环境修复过程中OPAHs类高毒中间产物积累问题奠定基础．
Abstract:
1-hydroxy-2-naphthoic acid was identified as one kind of OPAHs that was most easily accumulated during the process of bioremediation. It was toxic to almost all microorganisms, and inhibited successfully restoring PAHs polluted environment. In this study, P. stutzeri N2, which was selected for its special ability in our previous research, was taken as the object. The capability and characterization of 1-hydroxy-2-naphthoic acid degradation by P. stutzeri N2 was studied. At the same time, the intermediate metabolisms of 1-hydroxy-2-naphthoic acid degraded by P. stutzeri N2 were identified by GC-MS method. The results showed that P. stutzeri N2 could utilize 50 mg/mL 1-hydroxy-2-naphthoic acid as the sole carbon and energy resource. When octane was used as the co-carbon source, the growth increase and degradation rate of P. stutzeri N2 could be improved by 20%. Based on the analysis of nine degradation metabolites by P. stutzeri N2, decarboxylation was proposed as the main degradation pathway. This study can help lay a foundation for the problem of high-toxic OPAHs accumulation during the process of bioremediation of PAHs polluted environment.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目(51278405)；陕西省科学院应用基础研究项目（2013k-09）
收稿日期：2016-03-01 修改稿日期：2017-01-03
作者简介：王琰(1982－)，女，博士研究生，主要研究难降解有机物的生物降解理论．E-mail:900smile@163.com
更新日期/Last Update: 2017-03-16