[1]聂麦茜,贺美丽,张森爰,等.NY3 菌固定化及生物膜处理含油废水的研究.[J].西安建筑科技大学学报:自然科学版,2014,46(04):572-577.[doi:10.15986/j.1006-7930.2014.04.021]
 NIE Maiqian,HE Meili,ZHANG Senyuan,et al.Study on immobilization of NY3 and treatment of oil-bearing wastewater using bio-film[J].J.Xi’an Univ. of Arch. & Tech.:Natural Science Edition,2014,46(04):572-577.[doi:10.15986/j.1006-7930.2014.04.021]
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NY3 菌固定化及生物膜处理含油废水的研究.()
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西安建筑科技大学学报:自然科学版[ISSN:1006-7930/CN:61-1295/TU]

卷:
46
期数:
2014年04期
页码:
572-577
栏目:
出版日期:
2014-08-31

文章信息/Info

Title:
Study on immobilization of NY3 and treatment of oil-bearing wastewater using bio-film
文章编号:
1006-7930(2014)04-0572-06
作者:
聂麦茜1贺美丽1 张森爰1王 琰2聂红云1田晓婷1符海林1
(1.西安建筑科技大学环境与市政工程学院,陕西 西安 710055; 2.陕西省微生物研究所,陕西 西安 710043)
Author(s):
NIE Maiqian1 HE Meili1 ZHANG Senyuan1 WANG Yan2 NIE Hongyun1 TIAN Xiaoting1 FU Hailin1
( 1. School of Environmental and Municipal Engineering, Xi’an Univ. of Arch. & Tech., Xi’an 710055, China; 2. Shaanxi Province Institute of Microbiology, Xi’an 710043, China )
关键词:
铜绿假单胞菌NY3 菌固定化生物膜含油废水膜活性
Keywords:
Pseudomonas aeruginosa NY3 immobilized bio-film oil-bearing wastewater activity of bio-film
分类号:
X307
DOI:
10.15986/j.1006-7930.2014.04.021
文献标志码:
A
摘要:
利用嗜油菌NY3菌的固定化生物膜处理含油废水.用静态曝气法确定固定化时间、载体量、固定化液初始pH值.确定生物膜处理运行的HRT,并评估生物膜循环使用的可能性.结果表明,载体10 g/L,pH7.5~9.0,固定51 h,膜生物量达488.32 mg/g.其处理含油废水最佳HRT为6 h,出水油量在1.38~3.2 mg/L.废水处理后,将载体置于营养液中培养15 d,膜上NY3菌能将所吸附的油降解,使其恢复活性,并可循环利用.
Abstract:
Treatment of oil-bearing wastewater by using immobilized bio-film of NY3 strain was studied. The immobilization time, the optimum dosage of carrier, and the initial pH of immobilized culture for NY3 strain to be immobilized on surface of polyurethane foam carrier were studied by using static aeration. The optimum HRT was determined. Also the possibility of recycle of the bio-film was studied. The results showed that immobilized bio-film of NY3 strain could quickly form within 51 h under dosage of carrier was 10.0 g/L (culture medium),the initial pH of immobilized culture was 7.5~9. The maximum amount of biomass on bio-film reached 488.32 mg/g. The optimum HRT for treating oil-bearing wastewater is 6 h, and the concentrations of oil in effluent kept in range of 1.38-3.2 mg/L. After treatment, the biofilm collecting into culture medium without oil was incubated 15 d, NY3 strain on the bio-film degraded the adhered petroleum and the bio-film was recovered for recycling.

参考文献/References:

[1] 沈齐. 含油废水处理概况[J]. 北京石油化工学院学报, 2006, 14(3):35-38.

SHEN Qi. A profile of oily wastewater treatment[J]. Journal of Beijing Institute of Petro-chemical Technology, 2006, 14(3):35-38.

[2] 张文林, 李春利, 侯凯湖. 含油废水处理技术研究进展[J]. 化工进展, 2005, 24(11):1239-1243.

ZHANG Wenlin, LI Chunli, HOU Kaihu. The research progress of oily wastewater treatment technology[J]. Chemical Industry and Engineering Progress, 2005, 24(11):1239-1243.

[3] 李伟光 , 李欣, 朱文芳. 固定化生物活性炭处理含油废水的试验研究[J]. 哈尔滨商业大学学报:自然科学版, 2004, 20(2):187-190.

LI Weiguang, LI Xin, ZHU Wenfang. Study on treatment of oil wastewater with immobilized biological activated carbon[J]. Journal of Harbin University of Commerce: Natural Sciences Edition, 2004, 20(2):187-190.

[4] 湛美, 陈丹. 固定化菌剂处理含油废水的试验研究[J]. 环境保护科学, 2011, 37(5):23- 47.

ZHAN Mei, CHEN Dan. Experimental study on treatment of oil wastewater with immobilized agents[J]. Environmental Protection Science, 2011, 37(5):23- 47.

[5] ZHANG Zhengzhi, HOU Zhaowei, YANG Chunyu. etc. Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8[J]. Bioresource Technology, 102 :4111-4116.

[6] KUN Tong, ZHANG Yihe, GUO hua, ZHENG fang, et al. Treatment of heavy oil wastewater by a conventional activated sludge process coupled with an immobilized biological filter[J]. International Bio-deterioration & Biodegradation, 2013, 84:65-71.

[7] ABALOS A, VINAS M, SABATE J, et al. Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by pseudomonas aeruginosa AT10[J]. Biodegradation, 2004, 15:249-260.

[8] 杨二辉, 李大平. 高温优势菌生物膜法处理含油污水的中试[J]. 环境工程, 2004, 22(3):11-13.

YANG Erhui, LI Daping. Study on the treatment of oily wastewater with biofilm by high temperature dominant bacteria[J]. Environment Engineering, 2004, 22(3):11-13.

[9] 叶正芳, 倪晋仁. 污水处理的固定化微生物与游离微生物性能的比较[J]. 应用基础与工程科学学报, 2002, 10(14):2-7.

YE Zhengfang, NI Jinreng. Comparison of wastewater by immobilized microbial treatment and free microbial properties[J]. Journal of Basic Science and Engineering Application, 2002, 10(14):2-7.

[10] 赵昕, 汪严明, 叶正芳. 复合工程菌 B350 处理采油废水的反应动力学特征[J]. 中国给水排水, 2006, 22(17):70-73.

ZHAO Xi, WANG Yanming, YE Zhengfang. Reaction kinetics of treatment of wastewater B350 composite microbial engineering[J]. Chinese Water Supply and Drainage, 2006, 22(17): 70-73.

[11] XIN Zhao, YAN Ming, WANG Zhengfang. Oil field wastewater treatment in biological aerated filter by immobilized microorganisms.[J]. Process Biochemistry, 2006, 41, 1475–1483.

[12] 谷妮娜. 固定化微生物技术处理含油废水的研究[D]. 沈阳:东北大学, 2005.

GU Nina. Study on the Immobilized microorganism technology for oily wastewater treatment[D]. Shenyang: Northeastern University, 2005.

[13] NIE Maiqian, YIN Xihou, REN Chunyan, et al. Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3[J]. Biotechnology Advances, 2010, 28:635-643.

[14] 常虹, 聂麦茜, 葛碧洲. 铜绿假单胞菌 NY3 所产表面活性剂对原油降解的影响[J]. 环境工程学报, 2013, 7(2):771-776.

CHANG Hong, NIE Maiqian, GE Bizhou. Effects of surfactant on oil degradation by pseudomonas aeruginosa NY3[J]. Chinese Journal of Environmental Engineering, 2013, 7(2):771-776.

[15] MANOHAR S, KIM C K, KAREGOUDAR T B. Enhanced degradation of naphthalene by immobilization of pseudomonas sp. strain NGK1 in polyurethane foam[J]. APPI. Microbiol. Biotechnol, 2001, 55(3):311-316.

[16] 刘献玲. 固定化热带假丝酵母降解苯酚的实验与理论研究[D]. 天津:天津大学, 2006.

LIU Xieling. Study on the experiment and theoretical by immobilized candida tropical on phenol degradation[D]. Tianjin: Tianjin University, 2006.

[17] 屈常林, 高洪, 赵宝洪. 细菌生物被膜与抗生素耐药机制研究进展[D]. 动物医学进展, 2008, 29(3):86-90.

QU Changlin, GAO Hong, ZHAO Baohong. Bacterial biofilm and antibiotic resistance mechanisms is reviewed[D]. Progress in Veterinary Medicine, 2008, 29(3):86-90.

[18] 李彤, 庄辉. 细菌生物膜的研究进展[J]. 中华微生物学和免疫学杂志, 2002, 22(3):343-346.

LI Tong, ZHUANG Hui. The research progress of bacterial biofilm[J]. The Chinese Journal of Microbiology and Immunology, 2002, 22(3):343-346.

[19] 刘虹, 张兰英, 吴宇航. 泥炭对石油降解菌最佳固定化条件的研究[J]. 吉林化工学院学报, 2011, 28(1):26-28.

LIU Hong, ZHANG Lanying, WU Yuhang. Study on the best immobilized conditions of oil degradation bacteria about peat[J]. Journal of Ji Lin Institute of Chemical Technology, 2011, 28(1):26-28.

[20] 周林成, 李彦锋. 大孔载体固定化微生物处理污水研究[J]. 离子交换与吸附, 2007, 23(6):531-539.

ZHOU Lincheng, LI Yanfeng. Study on the oil wastewater treatment by macroporous carrier immobilized microorganism[J]. Ion Exchange And Odsorption, 2007, 23(6):531 - 539.

[21] LI P J, WANG X, SGNITTNI Fetal. Degradation of phenan-threne and pyrene in slurry reactors with immobilized bacteria zoogloea sp[J]. Environmental Engineering Science, 2005, 22(3):390-399.

[22] SCHOLZ M, MARTIN R J. Ecological equilibrium on biological activated carbon[J]. Water Science and Technology, 1997, 31(12):2959-2968.

[23] 周珊, 胡泽友, 喻景权. 竹炭固定化假单胞菌处理含酚废水的研究[J]. 高校化学工程学报, 2008, 22(5):889-894.

ZHOU Shan, HU Zeyou, YU Jingquan. Biodegradation of phenol wastewater by pseudomonas sp. immobilized on bamboo-carbon [J]. Journal of Chemical Engineering of Chinese Universities, 2008, 22(5):889-894.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(51278405); 陕西省国际科技合作重点项目(2012KW-25); 榆林市2011 年产学研合作项目
收稿日期:2013-12-26 修改稿日期:2014-07-25
作者简介: 聂麦茜(1960-),女,教授,从事微生物降解石油烃研究.E-mail:niemaiqian@xauat.edu.cn
更新日期/Last Update: 2015-10-06